I have started to work on three dimensional reconstruction using Amira 3.1, and now the new 4.1.1. on the Macintosh. A short movie provides a quick introduction to the way Amira works. Initially, a stack of histological sections is aligned, then individual organs are outlined on each section, and then the program makes a 3D model out of the outlines. These wire-mesh models can be rotated in the computer in any direction. Hence, for instance from cross sections, a dorsal view can be obtained.
The model shows only the anterior odontophore cartilages, the posterior odontophore cartilages, part of the circumoesophageal nerve ring, and the statocysts. The movie file below provides a quick insight about the technique. The cross sections are of Sinezona rimuloides from California.